Taq SSB (Active) Protéine

N° du produit ABIN2452212

Détail du produit

Antigène: Taq SSB

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Thermus aquaticus

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Attributs du produit: The absence of endonucleases and exonucleases was confirmed.

Pureté: > 95 % of protein determined by SDS-PAGE (CBB staining)

Information d'application

Indications d'application: Stabilizes single-stranded DNA in DNA replication,repair,and recombination

Commentaires:

Activity: Single-stranded DNA binding activity was confirmed.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 50 mM Tris-HCl ( pH 8. 0), 200 mM NaCl, 0. 1 mM dithiothreitol, 0. 5 mM EDTA, 50 % glycerol

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Référence

Dabrowski, Olszewski, Piatek, Kur: "Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification." dans: Protein expression and purification, Vol. 26, Issue 1, pp. 131-8, (2002) (PubMed).

Détail du antigène

Antigène: Taq SSB

Sujet: Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA. It plays important roles in DNA replication and recombination. Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa, same as that of the natural protein.

UniProt: Q9KH06