Nanog Protein (TAT tag)

N° du produit ABIN2468881

Protéine Recombinant Nanog exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Nanog Protein (TAT tag) (ABIN2468881)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: < 95 % by SDS-PAGE gel and HPLC analyses.

Détail du produit

Purification/Conjugué: Cette Nanog protéine est marqué à la TAT tag.

Séquence: MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR

Attributs du produit: Endotoxin level is less than 0.1 ng per ug (1EU/μg).

niveau d'endotoxine: Endotoxin level is less than 0.1 ng per ug (1 EU/μg).

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Conseil sur la manipulation: As with any protein, exposing Nanog-TAT recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Stock: -20 °C

Stockage commentaire: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Nanog-TAT is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Date de péremption: 24 months

Détail du antigène

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: Nanog

Sujet: Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.2 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide.

ID gène: 388112

OMIM: 74762336

UniProt: Q6NSW7

Pathways: Stem Cell Maintenance