MIA Protéine

N° du produit ABIN2648118

Cette protéine Recombinant MIA est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour MIA Protéine (ABIN2648118)

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 98 % pure

Détail du produit

Séquence: MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ

Attributs du produit: Purified recombinant Human MIA protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 µg/mL.

niveau d'endotoxine: < 0.1 ng per μg (1 EU/μg).

Information d'application

Indications d'application: Each Investigator should determine their own optimal working dilution for specific applications.

Commentaires:

Biological activity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/mL.

Buffer: Lyophilized from 15 mM Na3PO4, pH 7.2 with 75 mM NaCl.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Détail du antigène

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Autre désignation: MIA

Sujet: MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.
Alternative Names: Cartilage-derived retinoic acid-sensitive protein protein, CD-RAP protein, Melanoma Inhibitory Activity protein

Poids moléculaire: 12.2 kDa