MIA Protéine

N° du produit ABIN2648118

Détail du produit

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Séquence: MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ

Attributs du produit: Purified recombinant Human MIA protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 µg/mL.

Pureté: > 98 % pure

niveau d'endotoxine: < 0.1 ng per μg (1 EU/μg).

Information d'application

Indications d'application: Each Investigator should determine their own optimal working dilution for specific applications.

Commentaires:

Biological activity: The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/mL.

Buffer: Lyophilized from 15 mM Na3PO4, pH 7.2 with 75 mM NaCl.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Détail du antigène

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Autre désignation: MIA (MIA Produits)

Synonymes: CD-RAP Protein, Mia1 Protein, Cdrap Protein, zgc:194157 Protein, mia Protein, CDRAP Protein, Fdp Protein, MIA Protein, MIAL Protein, melanoma inhibitory activity Protein, otoraplin Protein, cilia and flagella associated protein 100 Protein, MIA Protein, Mia Protein, mia Protein, Otor Protein, CFAP100 Protein

Sujet: MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.
Alternative Names: Cartilage-derived retinoic acid-sensitive protein protein, CD-RAP protein, Melanoma Inhibitory Activity protein

Poids moléculaire: 12.2 kDa