CCL21 Protein (AA 24-134)

N° du produit ABIN2666610

Protéine Recombinant CCL21 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CCL21 Protein (AA 24-134) (ABIN2666610)

Antigène: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 24-134

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-hCCR7 transfectants in a dose dependent manner.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Autre désignation: CCL21

Sujet: CCL21 is a CC chemokine highly expressed in lymph nodes. CCL21 is only 32 % identical to CCL19 at the amino acid level, nevertheless, these chemokines bind to the same receptor CCR7. CCL21 has a unique extension of about 30 amino acids with two extra cysteine residues in its carboxyl terminus. CCL21 is expressed in synovial tissue in rhematoid arthritis (RA), and it has been suggested that CCL21, but not CCL19, induces human microvascular endothelial cell (HMVEC) migration and/or tube formation. In addition, it has been reported that sustained high concentrations of either soluble or immobilized CCL19 and CCL21 inhibit T cell proliferation and IL-2 secretion.

Poids moléculaire: The 111 amino acid recombinant protein has a predicted molecular mass of approximately 12 kDa. The DTT-reduced protein migrates at approximately 16 kDa and non-reduced protein at approximately 17 kDa by SDS-PAGE. The N-terminal amino acid is Ser.

Pathways: Regulation of Actin Filament Polymerization