CXCL11 Protein (AA 22-94)

N° du produit ABIN2666634

Protéine Recombinant CXCL11 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CXCL11 Protein (AA 22-94) (ABIN2666634)

Antigène: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 22-94

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-mCXCR3 transfectants in a dose dependent manner. The ED50 = 1 - 5 ng/m L.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Autre désignation: CXCL11

Sujet: CXCL11 is a CXC chemokine lacking the ELR (Glutamate-Leucine-Arginine) tripeptide motif, which is functionally and structurally related to CXCL9 and CXCL10. CXCL11, together with CXCL9 and CXCL10, specifically binds to the G protein-coupled receptor CXCR3 and is involved in chemotaxis of immune cells and angiogenesis. Expression of both CXCR3 and CXCL11 by the Th1-associated cytokine IFNγ creates an amplification loop of cell-mediated immune response between Th1 cells. CXCR7 has been identified as an alternate receptor for CXCL11 and is involved in growth, survival, and adhesion of tumor cells.

Poids moléculaire: The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.3 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein migrates at approximately 13 kDa by SDS-PAGE. The N-terminal amino acid is Meth