CXCL5 Protein (AA 45-118)

N° du produit ABIN2666646

Protéine Recombinant CXCL5 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CXCL5 Protein (AA 45-118) (ABIN2666646)

Antigène: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Multiplex Assay (MA)

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 45-118

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Autre désignation: CXCL5

Sujet: CXCL5 is an ELR+CXC chemokine characterized by a Glu-Leu-Arg (ELR) motif preceding the characteristic CXC sequence. CXCL5 chemoattracts and activates neutrophils during inflammation through the binding to CXCR2. CXCL5 occurs in different NH2- and COOH-truncated forms, and both NH2-terminal and COOH-terminal truncation result in an increase in neutrophil chemotactic potency. CXCL5(8-78) and CXCL5(9-78) induce a higher neutrophil influx in vivo compared with CXCL5(1-78). CXCL5 regulates the availability of binding sites for other ELR+ CXC chemokines released during inflammation, through its interaction with erythrocyte duffy antigen receptor (DARC). As a result, CXCL5 induces the increase of CXCL1 and CXCL2 in a model of pulmonary inflammation. Therefore, CXCL5 acts as a regulator of chemokine scavenging and pulmonary host defense to bacterial infection. Lung epithelial cells express CXCR2, consequently, CXCL5 may modulate neutrophil transepithelial and transendothelial migration. Mouse CXCL5 is the ortholog of human CXCL6.

Poids moléculaire: The 74 amino acid recombinant protein has a predicted molecular mass of approximately 7.98 kDa. The DTT-reduced protein and non-reduced protein migrates at approximately 10 kDa by SDS-PAGE. The N-terminal amino acid is Val.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity