CCL1 Protein (AA 24-92)

N° du produit ABIN2666951

Détail du produit

Antigène: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 24-92

Origine: Souris

Source: HEK-293 Cells

Application: Western Blotting (WB)

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: Bioactivity was measured by its property to chemoattract mouse BW.5147.G.1.4OUAR.1 cells in a dose dependent manner.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA, or HAS. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Autre désignation: CCL1 (CCL1 Produits)

Synonymes: I-309 Protein, P500 Protein, SCYA1 Protein, SISe Protein, TCA3 Protein, BF534335 Protein, Scya1 Protein, Tca-3 Protein, CCLi6 Protein, chemokine Protein, C-C motif chemokine ligand 1 Protein, chemokine (C-C motif) ligand 1 Protein, CCL1 Protein, Ccl1 Protein

Sujet: CCL1 was initially identified as a secreted protein derived from activated T cells. CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation, as a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and a higher concentration of CCL1 in bronchoalveolar lavage of asthmatics patients compared with normal controls. In addition, high levels of CCL1 have been detected in serum of patients with atopic dermatitis. Also, CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication showed that CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β in vitro, and this transcriptional upregulation is reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Also, antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.

Poids moléculaire: The 69 amino acid recombinant protein has a predicted molecular mass of approximately 7.7 kDa. The DTT-reduced protein migrates at approximately 16 kDa and non-reduced protein migrates at approximately 18-20 kDa by SDS-PAGE. The N-terminal amino acid is L