IL-22 Protein (AA 34-179)

N° du produit ABIN2667507

Protéine Recombinant IL-22 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-22 Protein (AA 34-179) (ABIN2667507)

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Immunofluorescence (IF)

Pureté: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 34-179

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: The ED50 is 0.062-0.177 ng/ml, corresponding to a specific activity of 1.61-0.56 X107 units/mg, as determined by a dose dependent stimulation of human Colo205 cells in production of IL-10.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Autre désignation: IL-22

Sujet: IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells.IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely Il-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79 % amino acid identity with mouse IL-22 and 25 % identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4 (+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.

Poids moléculaire: The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,880 Da. The DTT-reduced protein migrates at approximately 15kDa and the non-reduced protein migrates at approximately 14kDa by SDS-PAGE.