IL-22 Protein (AA 34-179)

N° du produit ABIN2667509

Cette protéine Recombinant IL-22 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL-22 Protein (AA 34-179) (ABIN2667509)

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Pureté: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Attributs du protein: AA 34-179

Stérilité: 0.22 μm filtered

niveau d'endotoxine:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: The ED50 is 0.095-0.265 ng/ml, corresponding to a specific activity of 1.05-0.38 X107 units/mg, as determined by a dose dependent stimulation of human Colo205 cells in production of IL-10.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Détail du antigène

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Autre désignation: IL-22

Sujet: IL-22, a cytokine structurally related to IL10, was originally identified in the mouse as a gene induced by IL9 in T cells and mast cells (1). IL-22 was initially designated as ILTIF (IL10-related T cell-derived inducible factor). Mouse IL-TIF consists of 179 amino acids, including four cysteins, and has a predicted molecular weight of 20 KD. IL-22 shows 22 % sequence identity with mouse IL-10 and belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B, and IL-29 (the latter 3 also known as IFN-λ) (3). IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1) (3). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22-binding receptor have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes (4). IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of the recently identified T helper 17 lineage of CD4(+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in dysregulated inflammation observed in autoimmune diseases (5).

Poids moléculaire: The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 16.8 kDaa. The DTT-reduced protein migrates at approximately 15 kDaa and the non-reduced protein migrates at approximately 13 kDaa by SDS-PAGE.