IL1F9 Protein (AA 18-169)

N° du produit ABIN2667525

Protéine Recombinant IL1F9 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour IL1F9 Protein (AA 18-169) (ABIN2667525)

Antigène: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Immunofluorescence (IF)

Pureté: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Détail du produit

Attributs du protein: AA 18-169

niveau d'endotoxine:

Less than 0.1 ng per μg of protein.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Biological activity: Measured by its ability to induce IL-8 production by human PBMCs.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Unopened vial can be stored at -20°C or -70°C.

Détail du antigène

Antigène: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Autre désignation: IL-36 gamma

Sujet: The IL-1 family is a group of cytokines comprised of 11 membranes, including recently renamed IL-36 cytokines, IL-36 α, β and γ (previously known as IL-1F6, IL-1F8 and IL-1F9). IL-36γ signals through the IL-1 receptor family members IL-1Rrp2 and IL-1RAcP and can activate downstream MAP kinases and transfection factor NF-kB. IL-36γ is synthesized without signal peptide and the secretion pathway is unclear. It has been shown that, like other IL-36 cytokines, N-terminal processing of IL-36γ can significantly increase its biological activity. The possible processing enzymes are currently unknown. IL-36γ also plays a significant role in immune response. Low level of IL-36 receptor IL-1Rrp can be detected on bone marrow-derived dendritic cells and CD4+ T cells. IL-36γ treatment can upgulate CD80, CD86 and MHC Class II, and proinflammatory cytokine producton in dendritic cells. IL-36γ has also been implicated in airway hyperesponsiveness. Intranasal administration of recombinant IL-36γ in mice leads to epithelial cell hypertrophy, cellular infiltration, and mucus production. IL-36γ expression in the lungs of mice can be upregulated by allergic inflammation.

Poids moléculaire: The 152 amino acid recombinant protein has a predicted molecular mass of approximately 17 kDa. The predicted N-terminal amino acid is Ser.