Nuclear Factor kappa B p50 (NFkB p50) (full length) Protéine

N° du produit ABIN2669406

Protéine Recombinant Nuclear Factor kappa B p50 (NFkB p50) exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Nuclear Factor kappa B p50 (NFkB p50) (full length) Protéine (ABIN2669406)

Antigène: Nuclear Factor kappa B p50 (NFkB p50)

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: ELISA, Gel Shift (GS)

Détail du produit

Attributs du protein: full length

Fonction: Recombinant NFkB p50 protein

Attributs du produit: Recombinant NFkB p50 protein was expressed from a full-length cDNA clone in E. coli (accession number P19838). Protein was purified by affinity chromatography, followed by gel filtration. NFkB p50, is a subunit of the NFkB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains, they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFkB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IkB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IkB proteins. This releases NFkB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes. Recombinant NFkB p50 is suitable for both gelshift and TransAM assays.

Purification: Protein was purified by affinity chromatography, followed by gel filtration.

Information d'application

Indications d'application: Recombinant NFkB p50 is suitable for both gelshift and TransAM assays. The standard curve for TransAM NFkB p50 was generated using the range of 10 - 0.156 ng of protein. This protein is not recommended for Western blotting. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Restrictions: For Research Use only

Stockage

Concentration: 100 ng/μL

Buffer: 5 μg of Recombinant NFkB p50 protein, 1X Protein Dilution Buffer (20 mM Tris-HCl, 0.2 M NaCl, 2 mM MgCl2 and 10 % glycerol) and 1 M DTT. Protein is supplied at 100 ng/ul in Protein Dilution Buffer.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: Nuclear Factor kappa B p50 (NFkB p50)

Autre désignation: NFkappaB p50

Sujet:

Protein Background: NFkB p50, is a subunit of the NFkB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains, they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFkB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IkB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IkB proteins. This releases NFkB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.

Short Description: Full length recombinant NFkB p50 protein was expressed in E. coli (accession number (P19838). Protein was purified by affinity chromatography, followed by gel filtration. The protein contains a His-Tag.

Aliases: p50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B