Histone H3.3 Protein (full length) (His tag)

N° du produit ABIN2669581

Cette protéine Recombinant Histone H3.3 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Histone H3.3 Protein (full length) (His tag) (ABIN2669581)

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Screening Assay (ScA), Activity Assay (AcA), Functional Studies (Func)

Pureté: >92 % pure by SDS-PAGE

Détail du produit

Attributs du protein: full length

Purification/Conjugué: Cette Histone H3.3 protéine est marqué à la His tag.

Fonction: Recombinant Histone H3.3 (Human)

Attributs du produit: Recombinant Histone H3.3 (Human) corresponds to the native histone sequence of mammalian histone H3 variant H3.3 and does not contain any amino acid substitutions or residue analogs. Histone H3.3 is known to be important for gene regulation. Recombinant Histone H3.3, Human consists of the full-length sequence of human Histone H3.3 variant (accession number NM_005324). Recombinant Histone H3.3, Human was generated in E. coli and has an observed molecular weight of 15.4 kDa. Recombinant Histone H3.3 can be used to study enzyme kinetics and screen inhibitors.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.4 μg/μL

Buffer: Full length recombinant Histone H3.3 (Human) is supplied in 25 mM Tris-HCl, pH 7.4, 150 mM NaCl and 20 % glycerol.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Stock: -80 °C

Stockage commentaire: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Détail du antigène

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Autre désignation: Histone H3.3

Sujet:

Protein Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.

Short Description: Recombinant Histone H3.3 (Human) corresponds to the native histone sequence of mammalian histone H3 variant H3.3 and does not contain any amino acid substitutions or residue analogs. Histone H3.3 is known to be important for gene regulation. Recombinant Histone H3.3 (Human) consists of the full-length sequence of human Histone H3.3 variant (accession number NM_005324). Recombinant Histone H3.3 (Human) was generated in E. coli cells and has an observed molecular weight of 15.4 kDa. The recombinant protein is >92 % pure by SDS-PAGE.

Poids moléculaire: 15.4 kDa