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IL-20 Protéine

Protéine IL-20 exprimée dans Escherichia coli (E. coli).
N° du produit ABIN2690584

Aperçu rapide pour IL-20 Protéine (ABIN2690584)

Antigène

Voir toutes IL-20 (IL20) Protéines
IL-20 (IL20) (Interleukin 20 (IL20))

Activité biologique

Active

Origine

  • 12
  • 6
  • 3
  • 1
Humain

Source

  • 15
  • 5
  • 1
Escherichia coli (E. coli)

Pureté

> 95 %, as determined by SDS-PAGE and HPLC
  • Specificité

    Optimized DNA sequence encoding Human Interleukin-20 mature chain was expressed in Escherichia Coli.

    Attributs du produit

    Native human Interleukin-20 generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa. Recombinant IL-20 is a disulfide-linked monomeric protein consisting of 135 amino acid residue subunits, and migrates as an approximately 18kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.

    Stérilité

    0.2 μm filtered

    niveau d'endotoxine

    Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
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  • Commentaires

    The ED(50) was determined by by a cell proliferation assay using human IL-20Ralpha and human IL-20Rbeta co-transfected murine BaF3 pro-B cells and was found to be less than 0.6 ng/mL, corresponding to a specific activity of 1.7 × 10 6 IU/mg.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

    Buffer

    PBS solution pH 7.5.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles.

    Stock

    -20 °C

    Stockage commentaire

    The lyophilized antibody is stable for at least 1 year from date of receipt at -20 °C. Upon reconstitution, this antibody can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months without detectable loss of activity.

    Date de péremption

    12 months
  • Antigène

    IL-20 (IL20) (Interleukin 20 (IL20))

    Autre désignation

    IL-20

    UniProt

    Q9NYY1

    Pathways

    Protein targeting to Nucleus
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