CA5B Protéine

N° du produit ABIN2713133

Protéine Recombinant CA5B exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CA5B Protéine (ABIN2713133)

Antigène: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Attributs du produit:

• Recombinant human Carbonic anhydrase 5B protein expressed in E. coli.
• Produced with end-sequenced ORF clone

niveau d'endotoxine: < 0.1 EU per μg protein as determined by LAL test

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Autre désignation: Carbonic Anhydrase 5b

Sujet: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.

Poids moléculaire: 33.8 kDa

NCBI Accession: NP_009151