Acetylcholinesterase Protein (AChE) (Myc-DYKDDDDK Tag)

N° du produit ABIN2714318

Protéine Recombinant Acetylcholinesterase exprimée dans HEK-293 Cells.

Aperçu rapide pour Acetylcholinesterase Protein (AChE) (Myc-DYKDDDDK Tag) (ABIN2714318)

Antigène: Acetylcholinesterase (AChE)

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Purification/Conjugué: Cette Acetylcholinesterase protéine est marqué à la Myc-DYKDDDDK Tag.

Attributs du produit:

• Recombinant human Acetylcholinesterase (transcript variant E4-E5) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: Acetylcholinesterase (AChE)

Autre désignation: Acetylcholinesterase

Sujet: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Poids moléculaire: 60.8 kDa

NCBI Accession: NP_056646

Pathways: Skeletal Muscle Fiber Development