Aconitase 1 Protein (ACO1) (Myc-DYKDDDDK Tag)

N° du produit ABIN2714323

Cette protéine Recombinant Aconitase 1 est exprimée dans HEK-293 Cells.

Aperçu rapide pour Aconitase 1 Protein (ACO1) (Myc-DYKDDDDK Tag) (ABIN2714323)

Antigène: Aconitase 1 (ACO1)

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Purification/Conjugué: Cette Aconitase 1 protéine est marqué à la Myc-DYKDDDDK Tag.

Attributs du produit:

• Recombinant human ACO1 / IREB1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: Aconitase 1 (ACO1)

Autre désignation: Aco1,ireb1

Sujet: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants

Poids moléculaire: 98.2 kDa

NCBI Accession: NP_002188

Pathways: Transition Metal Ion Homeostasis