GAPDH Protein (Myc-DYKDDDDK Tag)

N° du produit ABIN2721688

Cette protéine Recombinant GAPDH est exprimée dans HEK-293 Cells.

Aperçu rapide pour GAPDH Protein (Myc-DYKDDDDK Tag) (ABIN2721688)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Pureté: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Purification/Conjugué: Cette GAPDH protéine est marqué à la Myc-DYKDDDDK Tag.

Attributs du produit:

• Recombinant human GAPDH protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Commentaires:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Stockage

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: Gapdh

Sujet: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 35.9 kDa

NCBI Accession: NP_002037