IL27 Protéine
Aperçu rapide pour IL27 Protéine (ABIN2723689)
Antigène
Type de proteíne
Activité biologique
Origine
Source
Application
Pureté
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Specificité
- Optimal preservation of protein structure, post-translational modifications and functions.
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Attributs du produit
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- Recombinant human Interleukin-27 / IL27A protein expressed in HEK293.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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niveau d'endotoxine
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- The ED50 is 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.
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Indications d'application
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 20 mM NaH2PO4, pH 6, 0.60 M NaCl
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Stock
- -80 °C
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Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL27 (Interleukin-27 Subunit alpha (IL27))
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Autre désignation
- Interleukin-27,il27a
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Poids moléculaire
- 49 kDa
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NCBI Accession
- NP_663611
Antigène
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