MMP1
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 90 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD, Func, PI
Active
Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human MMP-1 (transcript variant 1) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 90 % as determined by SDS-PAGE and Coomassie blue staining
niveau d'endotoxine
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 ug/mL, 50% cleavage was achieved at an incubation time of approximately 25 minutes
MMP1
Origine: Cheval
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
SDS, WB, Imm, PC
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Conseil sur la manipulation
Resuspend the protein in the desired concentration in proper buffer
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.