Nerve Growth Factor Protein (NGF)

N° du produit ABIN2727244

Cette protéine Recombinant Nerve Growth Factor est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Nerve Growth Factor Protein (NGF) (ABIN2727244)

Antigène: Nerve Growth Factor (NGF)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human NGF protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

niveau d'endotoxine: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: determined by its ability to stimulate chick E9 DRG neurite outgrowth. ED50 is less than or equal to 1.0 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Conseil sur la manipulation: Resuspend the protein in the desired concentration in proper buffer

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: Nerve Growth Factor (NGF)

Autre désignation: Ngf

Sujet: This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene&aposs expression is associated with allergic rhinitis.

Poids moléculaire: 13.5 kDa

NCBI Accession: NP_002497

Pathways: Regulation of Cell Size