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Nerve Growth Factor Protein (NGF) (Transcript Variant A)

Protéine Recombinant Nerve Growth Factor exprimée dans Escherichia coli (E. coli).
N° du produit ABIN2727245

Aperçu rapide pour Nerve Growth Factor Protein (NGF) (Transcript Variant A) (ABIN2727245)

Antigène

Voir toutes Nerve Growth Factor (NGF) Protéines
Nerve Growth Factor (NGF)

Type de proteíne

Recombinant

Activité biologique

Active

Origine

  • 9
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Souris

Source

  • 26
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Escherichia coli (E. coli)

Application

Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Pureté

> 95 % as determined by SDS-PAGE and Coomassie blue staining
  • Attributs du protein

    Transcript Variant A

    Specificité

    Optimal preservation of protein structure, post-translational modifications and functions.

    Attributs du produit

    • Recombinant human NGF (transcript variant A) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.

    niveau d'endotoxine

    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

    Biological Activity Comment

    ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than or equal to 1.0 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.
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  • Indications d'application

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays

    Restrictions

    For Research Use only
  • Buffer

    Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

    Conseil sur la manipulation

    Resuspend the protein in the desired concentration in proper buffer

    Stock

    -80 °C

    Stockage commentaire

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Antigène

    Nerve Growth Factor (NGF)

    Autre désignation

    Ngf

    Poids moléculaire

    13.4 kDa

    NCBI Accession

    NP_038637

    Pathways

    Regulation of Cell Size
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