NUMB Protein (DYKDDDDK Tag)
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- Antigène Voir toutes NUMB Protéines
- NUMB (Numb Homolog (NUMB))
- Type de proteíne
- Recombinant
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Origine
- Humain
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Source
- Insect cells (Sf9)
- Purification/Conjugué
- Cette NUMB protéine est marqué à la DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Attributs du produit
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- Recombinant human NUMB (C-term flag tag) protein expressed in Sf9 cells.
- Produced with end-sequenced ORF clone
- Pureté
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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Numb Homolog (NUMB) (AA 1-135) protein (GST tag)
NUMB Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
Numb Homolog (NUMB) (AA 1-653) protein (Strep Tag)Crystallography grade NUMB Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Numb Homolog (NUMB) (AA 1-651) protein (Strep Tag)Crystallography grade NUMB Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Numb Homolog (NUMB) proteinNUMB Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
Numb Homolog (NUMB) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)NUMB Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Numb Homolog (NUMB) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)NUMB Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Indications d'application
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Commentaires
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 5 months from receipt of products under proper storage and handling conditions.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Antigène
- NUMB (Numb Homolog (NUMB))
- Autre désignation
- Numb (NUMB Produits)
- Synonymes
- C14orf41 Protein, S171 Protein, c14_5527 Protein, Nb Protein, NUMB, endocytic adaptor protein Protein, numb homolog (Drosophila) Protein, NUMB Protein, Numb Protein, numb Protein
- Sujet
- Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. [UniProtKB/Swiss-Prot Function]
- Poids moléculaire
- 66 kDa
- NCBI Accession
- NP_001005744
- Pathways
- Cell-Cell Junction Organization
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