NADK Protein (His tag)

N° du produit ABIN2745747

Cette protéine Recombinant NADK est produite dans Escherichia coli (E. coli).

Aperçu rapide pour NADK Protein (His tag) (ABIN2745747)

Antigène: NADK (NAD Kinase (NADK))

Type de proteíne: Recombinant

Origine: Mycobacterium tuberculosis

Source: Escherichia coli (E. coli)

Pureté: >98 % (SDS-PAGE)

Détail du produit

Purification/Conjugué: Cette NADK protéine est marqué à la His tag.

Fonction: NAD Kinase (M. tuberculosis) (rec.) (His)

Attributs du produit: NAD kinase (Mycobacterium tuberculosis) is fused at the N-terminus to a His-tag.

Biological Activity Comment: ~1.2 U/mg protein. (Substrate: NAD (+ ATP)) ~3.8 U/mg protein. (Substrate: NAD (+ poly(P)) One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinamide adenine dinucleotide phosphate (NADP) per min.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: 0.5 mg/mL (Lot specific)

Concentration: Lot specific

Buffer: In 10 mM TRIS at pH 8.0, containing 1 mM MgCl2, 0.5 mM EDTA, 1 mM DTT and 1 mM PMSF.

Conseil sur la manipulation: After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.

Stock: -20 °C,-80 °C

Stockage commentaire:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: NADK (NAD Kinase (NADK))

Autre désignation: NAD Kinase

Sujet:

Probable Inorganic Polyphosphate/ATP-NAD kinase, Poly(P)/ATP NAD Kinase 1, ppnK1, EC 2.7.1.23

NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+

Poids moléculaire: ~33.0kDa

UniProt: P0A5S6