CIART (AA 1-375) protein (Strep Tag)

N° du produit ABIN3134982

Cette protéine Recombinant CIART est exprimée dans Cell-free protein synthesis (CFPS).

Aperçu rapide pour CIART (AA 1-375) protein (Strep Tag) (ABIN3134982)

Antigène: CIART

Type de proteíne: Recombinant

Origine: Souris

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Pureté: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Classe de qualité: custom-made

Détail du produit

Attributs du protein: AA 1-375

Purification/Conjugué: Strep Tag

Séquence: MDSPSSVSSY SSSSLSPSFS TSSVNSDFSF PSDNEREGKG THELRPDTVG QRGGSRPSPG PIRCRHRPRV SSNQHTAPHL EQQGSEVKRS RDGEQETSLN TQGCTTEGDL LFAQKCKELQ GFIRPLTDLL NGLKMGRFDR GLSSFQQSVA MDRIQRIVGV LQKPQMGERY LGTLLQVEGM LKTWFPHIAA QKSSSGGSRH QISKHFPSHH GDPGAASPAP LLEKMGQTQL GHLVLKPKQP WHLTGWPAMN LTWIHSTPIC NPPLSSQGSA SGHSPIGTGA SIGVILVLQK GGQPFTHSAP GTPVPPTPLS PVVPGDLKKL PGEEPRCHSL PVTLPSDWSC ILCPPVLPTT DREMTKGHPE PQMTSHPPVA PDPQP
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: 12 months

Détail du antigène

Antigène: CIART

Sujet: Circadian-associated transcriptional repressor (ChIP-derived repressor of network oscillator) (Chrono) (Computationally highlighted repressor of the network oscillator),FUNCTION: Transcriptional repressor which forms a negative regulatory component of the circadian clock and acts independently of the circadian transcriptional repressors: CRY1, CRY2 and BHLHE41. In a histone deacetylase-dependent manner represses the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Abrogates the interaction of BMAL1 with the transcriptional coactivator CREBBP and can repress the histone acetyl-transferase activity of the CLOCK-BMAL1 heterodimer, reducing histone acetylation of its target genes. Rhythmically binds the E-box elements (5'-CACGTG-3') on circadian gene promoters and its occupancy shows circadian oscillation antiphasic to BMAL1. Interacts with the glucocorticoid receptor (NR3C1) and contributes to the repressive function in the glucocorticoid response. {ECO:0000269|PubMed:24385426, ECO:0000269|PubMed:24736997, ECO:0000269|PubMed:24737000}.

Poids moléculaire: 40.4 kDa

UniProt: Q3TQ03