Protein L Protein (His tag)

N° du produit ABIN2181671

Protéine Recombinant Protein L exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour Protein L Protein (His tag) (ABIN2181671)

Antigène: Protein L

Type de proteíne: Recombinant

Origine: Peptostreptococcus magnus

Source: Escherichia coli (E. coli)

Pureté: >95 % as determined by SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette Protein L protéine est marqué à la His tag.

Marque: MABSol®

Attributs du produit: Recombinant Protein L Protein is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 36-38 kDa polypeptide .Protein L Protein can bind to VL-Kappa.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Buffer: 100 mM NaH2PO4, pH 8.5

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Référence

Martinović, Andjelković, Klobučar, Černigoj, Vidič, Lučić, Pavelić, Josić: "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum." dans: Electrophoresis, Vol. 38, Issue 22-23, pp. 2909-2913, (2017) (PubMed).

Détail du antigène

Antigène: Protein L

Sujet: Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

ID gène: 17