CD112R (AA 28-165) protein (Fc Tag)

N° du produit ABIN5564502

Cette protéine Recombinant CD112R est produite dans HEK-293 Cells.

Aperçu rapide pour CD112R (AA 28-165) protein (Fc Tag) (ABIN5564502)

Antigène: CD112R

Type de proteíne: Recombinant

Origine: Souris

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Pureté: >90 % (SDS-PAGE)

Détail du produit

Attributs du protein: AA 28-165

Purification/Conjugué: Fc Tag

Fonction: CD112R (mouse):Fc (human)(rec.)

Réactivité croisée: Souris

Attributs du produit: The extracellular domain of mouse CD112R (aa 28-165) is fused at the C-terminus to the Fc portion of human IgG1.

niveau d'endotoxine: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Binds to the ligand mouse CD112 (PVRL2, Nectin-2) with an EC50 of 5nM.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Conseil sur la manipulation: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Stock: 4 °C,-20 °C

Stockage commentaire:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CD112R

Sujet:

CD112 Receptor, Poliovirus Receptor-related Immunoglobulin Domain-containing Protein, Transmembrane Protein PVRIG

CD112 (also called Nectin-2 or poliovirus receptor-related 2 protein) is a type I transmembrane glycoprotein and a member of the Ig gene superfamily (IGSF). CD112/Nectin-2 functions as an adhesion molecule involved in the formation of cell junctions and interactions with other Nectin-family molecules. Nectin-2 is a also a surface marker of some endothelial cells and regulates migration, proliferation and angiogenesis. CD112 functions as an immune checkpoint regulator by modulating T cell signaling, either as a costimulator of T cell function, or as a coinhibitor of T cells proliferation by binding to the receptors CD226 or TIGIT (T cell Ig and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain), respectively. The binding of CD112 ligand to these receptors is weak compared to CD155. Recently, a new receptor of CD112, called CD112R or PVRIG (Poliovirus receptor-related immunoglobulin domain-containing protein) has been characterized, showing high affinity to CD112. CD112R is a new coinhibitory receptor of the PVR family for human T cells, inhibiting TCR-mediated signals upon binding to CD112/Nectin-2. CD112R is preferentially expressed in T lymphocytes and NK cells. Cancer cells evade immune responses as a result of an immunosuppressive tumor microenvironment and CD112R, by playing a role in the regulation of the immune system, could be a new attractive cancer immunotherapy target.

Poids moléculaire: ~48-50kDa (SDS-PAGE)