OspA Protéine

N° du produit ABIN5624575

N° du produit (Fournisseur): 000-001-c13

Cette protéine Recombinant OspA est produite dans Escherichia coli (E. coli).

Aperçu rapide pour OspA Protéine (ABIN5624575)

Antigène: OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))

Type de proteíne: Recombinant

Origine: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 000-001-c13

Fournisseur: Rockland

Fonction: OspA Control Protein

Purification: OspA is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspA and was estimated to be greater than 90% pure.

Stérilité: Sterile filtered

Information d'application

Indications d'application:

Application Note: OspA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 70.5 kDa for OspA-MBP, (28.1 kDa for OspA and 42.4 for MBP) in size corresponding to OspA by Western blotting in the appropriate cell lysate or extract. Outer-Surface Protein A has been tested in SDS-page and western blot.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Date de péremption: 6 months

Détail du antigène

Antigène: OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))

Autre désignation: OspA

Classe de substances: Bacteria Protein

Sujet:

Synonyms: Outer surface protein A, Borrelia burgdorferi OspA, control protein

Background: Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

ID gène: 1194357

NCBI Accession: WP_010890378

UniProt: P0CL66