ErpN/OspE Protéine

N° du produit ABIN5624582

N° du produit (Fournisseur): 000-001-c10

Cette protéine Recombinant ErpN/OspE est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour ErpN/OspE Protéine (ABIN5624582)

Antigène: ErpN/OspE

Type de proteíne: Recombinant

Origine: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 000-001-c10

Fournisseur: Rockland

Fonction: ErpN/OspE Control Protein

Purification: ErpN/OspE is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified ErpN/OspE and was estimated to be greater than 90% pure.

Stérilité: Sterile filtered

Information d'application

Indications d'application:

Application Note: ErpN/OspE is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 59.5 kDa for OspE-MBP, (17.1 kDa for OspE and 42.4 for MBP) in size corresponding to ErpN/OspE by Western blotting in the appropriate cell lysate or extract. ErpN/OspE protein has been tested in SDS-page and western blot.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Date de péremption: 6 months

Détail du antigène

Antigène: ErpN/OspE

Sujet:

Synonyms: ErpN, ErpA, outer surface protein E, Borrelia burgdorferi OspE, ospE/F-Related Protein N, control protein

Background: ErpN (OspE/F-Related Protein N), is from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. Erp proteins from Borrelia burgdorferi are postulated to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, indicative of Erp synthesis during the initial stages of vertebrate infection. It is postulated that surface-exposed Erp proteins could facilitate interactions with host tissues during the establishment of vertebrate infection. Lyme disease proteins are ideal for researchers interested in immunology, neurology, and rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

ID gène: 1194664

NCBI Accession: WP_010883865

UniProt: Q9S060