YBX3/DBPA Protéine

N° du produit ABIN5624583

N° du produit (Fournisseur): 000-001-b98

Protéine Recombinant YBX3/DBPA exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour YBX3/DBPA Protéine (ABIN5624583)

Antigène: YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))

Type de proteíne: Recombinant

Origine: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 000-001-b98

Fournisseur: Rockland

Fonction: DbpA Control Protein

Purification: DbpA is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Decorin Binding Protein A and was estimated to be greater than 90% pure.

Stérilité: Sterile filtered

Information d'application

Indications d'application:

Application Note: DbpA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 60.9 kDa for DbpA-MBP, (18.5 kDa for DbpA and 42.4 kDa for MBP) in size corresponding to DbpA by Western blotting in the appropriate cell lysate or extract. Decorin Binding Protein A has been tested in SDS-page and western blot.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Date de péremption: 6 months

Détail du antigène

Antigène: YBX3/DBPA (YBX3) (Y Box Binding Protein 3 (YBX3))

Autre désignation: DbpA

Sujet:

Synonyms: Decorin-binding Protein A, Borrelia burgdorferi dbpA, control protein, DbpA Control Protein

Background: DbpA, or Decorin Binding Protein A is from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. DbpA from other microbial organisms such as E. coli (ATP-dependent RNA helicase DbpA) are significantly different. The spirochete migrates from the tick midgut during tick feeding to tick salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize in mammalian tissues. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

ID gène: 1194347

NCBI Accession: WP_010890380

UniProt: O50917