CRASP-1 Protéine

N° du produit ABIN5624584

N° du produit (Fournisseur): 000-001-c18

Cette protéine Recombinant CRASP-1 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour CRASP-1 Protéine (ABIN5624584)

Antigène: CRASP-1

Type de proteíne: Recombinant

Origine: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 000-001-c18

Fournisseur: Rockland

Fonction: Crasp-1 Control Protein

Purification: Crasp1 is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Crasp1 and was estimated to be greater than 90% pure.

Stérilité: Sterile filtered

Information d'application

Indications d'application:

Application Note: Crasp1 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect bands at 69.3 kDa for CRASP-1-MBP, (26.9 kDa for CRASP-1 and 42.4 kDa for MBP) in size corresponding to Crasp1 by Western blotting in the appropriate cell lysate or extract. Complement Regulator-Acquiring Surface Protein 1 was tested in SDS-page and western blot.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Date de péremption: 6 months

Détail du antigène

Antigène: CRASP-1

Autre désignation: Crasp1

Sujet:

Synonyms: control protein, Complement regulator acquiring protein 1, Borrelia burgdorferi CRASP-1

Background: CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

ID gène: 1194383

NCBI Accession: WP_010890397

UniProt: Q66ZC1