Collagen IV Protéine

N° du produit ABIN5624596

N° du produit (Fournisseur): 009-001-106

Cette protéine Native Collagen IV est produite dans Human.

Aperçu rapide pour Collagen IV Protéine (ABIN5624596)

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Type de proteíne: Native

Origine: Humain

Source: Human

Application: ELISA, Immunohistochemistry (IHC), SDS-PAGE (SDS), Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 009-001-106

Fournisseur: Rockland

Fonction: Human Collagen Type IV

Attributs du produit: Type IV collagen, collagen 4, human collagen, Collagen alpha-4 (IV) chain

Purification: This product has been prepared from human placenta and is chromatographically and immunologically pure.  This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  This product reacts with anti-Collagen Type IV.  Reaction with Rockland's anti-Collagen I, II, III, V or VI is negligible.

Stérilité: Sterile filtered

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Collagen type IV has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.5 M Acetic Acid

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 6 months

Détail du antigène

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Autre désignation: COLLAGEN IV

Sujet: Background: Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.

UniProt: P02462