SMURF2 Protein (AA 1-748, full length) (GST tag)
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- Antigène Voir toutes SMURF2 Protéines
- SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))
- Type de proteíne
- Recombinant
- Attributs du protein
- full length, AA 1-748
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette SMURF2 protéine est marqué à la GST tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MSNPGGRRNG PVKLRLTVLC AKNLVKKDFF RLPDPFAKVV VDGSGQCHST DTVKNTLDPK WNQHYDLYIG KSDSVTISVW NHKKIHKKQG AGFLGCVRLL SNAINRLKDT GYQRLDLCKL GPNDNDTVRG QIVVSLQSRD RIGTGGQVVD CSRLFDNDLP DGWEERRTAS GRIQYLNHIT RTTQWERPTR PASEYSSPGR PLSCFVDENT PISGTNGATC GQSSDPRLAE RRVRSQRHRN YMSRTHLHTP PDLPEGYEQR TTQQGQVYFL HTQTGVSTWH DPRVPRDLSN INCEELGPLP PGWEIRNTAT GRVYFVDHNN RTTQFTDPRL SANLHLVLNR QNQLKDQQQQ QVVSLCPDDT ECLTVPRYKR DLVQKLKILR QELSQQQPQA GHCRIEVSRE EIFEESYRQV MKMRPKDLWK RLMIKFRGEE GLDYGGVARE WLYLLSHEML NPYYGLFQYS RDDIYTLQIN PDSAVNPEHL SYFHFVGRIM GMAVFHGHYI DGGFTLPFYK QLLGKSITLD DMELVDPDLH NSLVWILEND ITGVLDHTFC VEHNAYGEII QHELKPNGKS IPVNEENKKE YVRLYVNWRF LRGIEAQFLA LQKGFNEVIP QHLLKTFDEK ELELIICGLG KIDVNDWKVN TRLKHCTPDS NIVKWFWKAV EFFDEERRAR LLQFVTGSSR VPLQGFKALQ GAAGPRLFTI HQIDACTNNL PKAHTCFNRI DIPPYESYEK LYEKLLTAIE ETCGFAVE
- Purification
- SDS-PAGE
- Pureté
- > 90 %
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- Discover our top product SMURF2 Protéine
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SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 1-748) protein (Strep Tag)
Crystallography grade SMURF2 Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 1-748) protein (Strep Tag)Crystallography grade SMURF2 Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 1-748), (full length) protein (His tag)SMURF2 Origine: Humain Hôte: Levure Recombinant > 90 % SDS
SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) protein (Myc-DYKDDDDK Tag)SMURF2 Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Stock
- -80 °C,4 °C,-20 °C
- Stockage commentaire
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Antigène
- SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))
- Autre désignation
- SMUF2 (SMURF2 Produits)
- Synonymes
- SMURF2 Protein, 2810411E22Rik Protein, AI558114 Protein, AI649275 Protein, RGD1310067 Protein, wu:fb82a11 Protein, wu:fc41c02 Protein, SMAD specific E3 ubiquitin protein ligase 2 Protein, SMAD specific E3 ubiquitin protein ligase 2 S homeolog Protein, SMURF2 Protein, smurf2 Protein, Smurf2 Protein, smurf2.S Protein
- Sujet
- E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
- Poids moléculaire
- 113.54 kDa
- UniProt
- Q9HAU4
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