Streptavidin Protein (AA 37-159) (His tag)

N° du produit ABIN5853015

Cette protéine Recombinant Streptavidin est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Streptavidin Protein (AA 37-159) (His tag) (ABIN5853015)

Antigène: Streptavidin

Type de proteíne: Recombinant

Origine: Streptomyces

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 90% by SDS-PAGE

Détail du produit

Attributs du protein: AA 37-159

Purification/Conjugué: Cette Streptavidin protéine est marqué à la His tag.

Séquence: AEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Streptavidin

Sujet: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 15.6 kDa (148aa) confirmed by MALDI-TOF