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CS (AA 28-466) protein (His tag)

Cette protéine Recombinant est exprimée dans Escherichia coli (E. coli).
N° du produit ABIN5853870

Aperçu rapide pour CS (AA 28-466) protein (His tag) (ABIN5853870)

Antigène

CS

Type de proteíne

Recombinant

Origine

Humain

Source

  • 3
  • 2
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Pureté

> 90 % by SDS - PAGE
  • Attributs du protein

    AA 28-466

    Purification/Conjugué

    His tag

    Séquence

    MGSSHHHHHH SSGLVPRGSH MGSASSTNLK DILADLIPKE QARIKTFRQQ HGKTVVGQIT VDMMYGGMRG MKGLVYETSV LDPDEGIRFR GFSIPECQKL LPKAKGGEEP LPEGLFWLLV TGHIPTEEQV SWLSKEWAKR AALPSHVVTM LDNFPTNLHP MSQLSAAVTA LNSESNFARA YAQGISRTKY WELIYEDSMD LIAKLPCVAA KIYRNLYREG SGIGAIDSNL DWSHNFTNML GYTDHQFTEL TRLYLTIHSD HEGGNVSAHT SHLVGSALSD PYLSFAAAMN GLAGPLHGLA NQEVLVWLTQ LQKEVGKDVS DEKLRDYIWN TLNSGRVVPG YGHAVLRKTD PRYTCQREFA LKHLPNDPMF KLVAQLYKIV PNVLLEQGKA KNPWPNVDAH SGVLLQYYGM TEMNYYTVLF GVSRALGVLA QLIWSRALGF PLERPKSMST EGLMKFVDSK SG
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  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1  mM DTT

    Stock

    4 °C,-20 °C,-80 °C

    Stockage commentaire

    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Antigène

    CS

    Sujet

    Citrate synthase (CS) is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. It is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. Recombinant human CS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

    Poids moléculaire

    51.4kDa (462aa)

    NCBI Accession

    NP_004068

    UniProt

    O75390
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