Topoisomerase I Protein (His tag)

N° du produit ABIN6126489

Cette protéine Recombinant Topoisomerase I est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Topoisomerase I Protein (His tag) (ABIN6126489)

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS)

Pureté: > 80 %

Détail du produit

Purification/Conjugué: Cette Topoisomerase I protéine est marqué à la His tag.

Fonction: Mouse Topoisomerase I (TOP1) Protein

Séquence: MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK DKDKDREKSK HSNSEHKDSE KKHKEKEKTK HKDGSSEKHK DKHKDRDKER RKEEKIRAAG DAKIKKEKEN GFSSPPRIKD EPEDDGYFAP PKEDIKPLKR LRDEDDADYK PKKIKTEDIK KEKKRKSEEE EDGKLKKPKN KDKDKKVAEP DNKKKKPKKE EEQKWKWWEE ERYPEGIKWK FLEHKGPVFA PPYEPLPESV KFYYDGKVMK LS

Biological Activity Comment: Not tested

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.

Stock: 4 °C,-80 °C

Stockage commentaire: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Autre désignation: Topoisomerase I

Classe de substances: Viral Protein

Poids moléculaire: Calculated MW: 34.1 kDa
Observed MW (SDS-PAGE): 38 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

ID gène

21969

UniProt: Q04750

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance