NAD Synthetase protein (His tag)

N° du produit ABIN6253505

Protéine Recombinant exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour NAD Synthetase protein (His tag) (ABIN6253505)

Antigène: NAD Synthetase

Type de proteíne: Recombinant

Origine: Mycobacterium tuberculosis

Source: Escherichia coli (E. coli)

Pureté: >98 % (SDS-PAGE)

Détail du produit

Purification/Conjugué: His tag

Fonction: NAD Synthetase (M. tuberculosis) (rec.) (His)

Specificité: NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.

Attributs du produit: NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.

Biological Activity Comment: ~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: 0.5 mg/mL (Lot specific)

Concentration: Lot specific

Buffer: In 20 mM TRIS-HCl, pH 7.8.

Conseil sur la manipulation: After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.

Stock: -20 °C,-80 °C

Stockage commentaire:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: NAD Synthetase

Sujet:

NADE, Glutamine-dependent NAD(+) Synthetase, EC 6.3.5.1

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate

Poids moléculaire: ~75.0kDa

UniProt: P0A5L6