NAD Synthetase protein (His tag)
Origine: Mycobacterium tuberculosis
Hôte: Escherichia coli (E. coli)
Recombinant
>98 % (SDS-PAGE)
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- Antigène
- NAD Synthetase
- Type de proteíne
- Recombinant
- Origine
- Mycobacterium tuberculosis
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- His tag
- Fonction
- NAD Synthetase (M. tuberculosis) (rec.) (His)
- Specificité
- NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
- Attributs du produit
- Protein. NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 20 mM TRIS-HCl, pH 7.8. Purity: >98 % (SDS-PAGE). NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate
- Pureté
- >98 % (SDS-PAGE)
- Biological Activity Comment
- ~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. In 20 mM TRIS-HCl, pH 7.8.
- Conseil sur la manipulation
- After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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- Antigène
- NAD Synthetase
- Sujet
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Alternate Names/Synonyms: NADE, Glutamine-dependent NAD(+) Synthetase, EC 6.3.5.1
Product Description: NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate
- UniProt
- P0A5L6
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