NME2 Protein (AA 1-152)
Aperçu rapide pour NME2 Protein (AA 1-152) (ABIN6387709)
Antigène
Voir toutes NME2 ProtéinesType de proteíne
Activité biologique
Origine
Source
Application
Pureté
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Attributs du protein
- AA 1-152
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Séquence
- MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE
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Biological Activity Comment
- Specific activity is > 1,800unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
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Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)
custom-made NME2 Origine: Souris Hôte: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)custom-made NME2 Origine: Humain Hôte: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (GST tag)NME2 Origine: Humain Hôte: Wheat germ Recombinant ELISA, WB, AP, AA
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His-IF2DI Tag)NME2 Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His-IF2DI Tag)NME2 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Nucleoside diphosphate kinase B (NME2) (AA 1-152) proteinNME2 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Commentaires
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- NME2 (Nucleoside diphosphate kinase B (NME2))
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Autre désignation
- NM23-H2/NME2
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Sujet
- NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 17.2 kDa (152aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001018149
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UniProt
- P22392
Antigène
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