AKR1C1 Protein (AA 1-323)

N° du produit ABIN6387732

Cette protéine Recombinant AKR1C1 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour AKR1C1 Protein (AA 1-323) (ABIN6387732)

Antigène: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 95% by SDS-PAGE

Détail du produit

Attributs du protein: AA 1-323

Séquence: MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 500pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.5) containing 0.1M NaCl, 20 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))

Autre désignation: Aldo-keto Reductase 1C1/AKR1C1

Sujet: AKR1C1 also known as Aldo-keto reductase family 1 member C1, is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant human AKR1C1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 36.7 kDa (323aa)

NCBI Accession: NP_001344

UniProt: Q04828

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process