AKR1C4 Protein (AA 1-323)

N° du produit ABIN6387734

Cette protéine Recombinant AKR1C4 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour AKR1C4 Protein (AA 1-323) (ABIN6387734)

Antigène: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 90% by SDS-PAGE

Détail du produit

Attributs du protein: AA 1-323

Séquence: MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer(  pH 8.5) containing 0.1M NaCl, 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Autre désignation: Aldo-keto Reductase 1C4/AKR1C4

Sujet: AKR1C1 also known as Aldo-keto reductase family 1 member C4, belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. Recombinant human AKR1C4 protein was expressed in E. coli and purified by using conventional chromatography.

Poids moléculaire: 37 kDa (323aa)

NCBI Accession: NP_001809

UniProt: P17516

Pathways: Steroid Hormone Biosynthesis