AKR1C4 Protein (AA 1-323)

N° du produit ABIN6387734

Détail du produit

Antigène: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-323

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Séquence: MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer(  pH 8.5) containing 0.1M NaCl, 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Autre désignation: Aldo-keto reductase family 1 member C4 (AKR1C4 Produits)

Synonymes: 3-alpha-HSD Protein, C11 Protein, CDR Protein, CHDR Protein, DD-4 Protein, DD4 Protein, HAKRA Protein, aldo-keto reductase family 1 member C4 Protein, AKR1C4 Protein

Sujet: AKR1C1 also known as Aldo-keto reductase family 1 member C4, belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. Recombinant human AKR1C4 protein was expressed in E.coli and purified by using conventional chromatography.

Poids moléculaire: 37kDa (323aa)

NCBI Accession: NP_001809

UniProt: P17516

Pathways: Steroid Hormone Biosynthesis