His-Streptavidin (AA 25-183) protein (His tag)

N° du produit ABIN666648

Cette protéine Recombinant est exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour His-Streptavidin (AA 25-183) protein (His tag) (ABIN666648)

Antigène: His-Streptavidin

Type de proteíne: Recombinant

Origine: Streptomyces avidinii

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 % by SDS - PAGE

Détail du produit

Attributs du protein: AA 25-183

Purification/Conjugué: His tag

Attributs du produit: His-Streptavidin, 25-183aa, Streptomyces avidinii, His tagged, Recombinant, E.coli

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid in 20mM Tris pH 7.5

Stock: 4 °C

Référence

Lanz, Masi, Porciello, Cohnen, Cipria, Prakaash, Bálint, Raggiaschi, Galgano, Cole, Lepore, Dushek, Dustin, Sansom, Kalli, Acuto: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." dans: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).

Détail du antigène

Antigène: His-Streptavidin

Sujet: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2. NCBI no.: CAA27265, P22629

Poids moléculaire: 17 kDa (167 aa), confirmed by MALDI-TOF.