Streptavidin Protein (AA 25-183) (His tag)

N° du produit ABIN666648

Cette protéine Recombinant Streptavidin est exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour Streptavidin Protein (AA 25-183) (His tag) (ABIN666648)

Antigène: Streptavidin

Type de proteíne: Recombinant

Origine: Streptomyces

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 25-183

Purification/Conjugué: Cette Streptavidin protéine est marqué à la His tag.

Séquence: DP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ

Attributs du produit: His-Streptavidin, 25-183aa, Streptomyces avidinii, His tagged, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5)

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Référence

Lanz, Masi, Porciello, Cohnen, Cipria, Prakaash, Bálint, Raggiaschi, Galgano, Cole, Lepore, Dushek, Dustin, Sansom, Kalli, Acuto: "Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation." dans: Cell reports, Vol. 36, Issue 2, pp. 109375, (2022) (PubMed).

Détail du antigène

Antigène: Streptavidin

Sujet: Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin, the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 17.5 kDa (167aa) confirmed by MALDI-TOF