PPIA Protein (AA 1-165) (His tag)

N° du produit ABIN666698

Cette protéine Recombinant PPIA est produite dans Escherichia coli (E. coli).

Aperçu rapide pour PPIA Protein (AA 1-165) (His tag) (ABIN666698)

Antigène: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-165

Purification/Conjugué: Cette PPIA protéine est marqué à la His tag.

Séquence: MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE

Attributs du produit: Cyclophilin A, 1-165 aa , Human, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 650nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 mM NaCl, 0.5 mM DTT, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Autre désignation: Cyclophilin A/PPIA

Sujet: Cyclophilin A (also known as Peptidylprolyl isomerase A, PPIA) encodes a member of the peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. Cyclophilin A is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity. Recombinant human PPIA, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 20kDa (185aa) confirmed by MALDI-TOF

NCBI Accession: NP_066953