Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1) (AA 1-316) (Active) Protéine

N° du produit ABIN666726

Cette protéine Recombinant Aldo-Keto Reductase Family 1 Member B10-Like 1 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1) (AA 1-316) (Active) Protéine (ABIN666726)

Antigène: Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Enzyme Activity Assay (EAA), SDS-PAGE (SDS)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-316

Séquence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF

Attributs du produit: Aldose reductase, 1-316aa, Human, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is >800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Aldo-Keto Reductase Family 1 Member B10-Like 1 (AKR1B10L1)

Autre désignation: Aldo-keto reductase 1B1/AKR1B1

Sujet: Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 35.8 kDa (316aa) confirmed by MALDI-TOF

NCBI Accession: NP_001619