CERT1 (AA 347-598) protein (His tag)
Aperçu rapide pour CERT1 (AA 347-598) protein (His tag) (ABIN667012)
Antigène
Type de proteíne
Origine
Source
Application
Pureté
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Attributs du protein
- AA 347-598
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Purification/Conjugué
- His tag
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Séquence
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LH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF -
Attributs du produit
- CERT, 347-598aa, Human, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol 0.1M NaCl
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CERT1
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Sujet
- CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 32.9 kDa (290aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_112729
Antigène
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