Aldo-Keto Reductase 1B10/AKR1B10 (AA 1-316) (Active) Protéine

N° du produit ABIN667103

Cette protéine Recombinant Aldo-Keto Reductase 1B10/AKR1B10 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Aldo-Keto Reductase 1B10/AKR1B10 (AA 1-316) (Active) Protéine (ABIN667103)

Antigène: Aldo-Keto Reductase 1B10/AKR1B10

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Enzyme Activity Assay (EAA), SDS-PAGE (SDS)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-316

Séquence: MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY

Attributs du produit: AKR1B10, 1-316 aa, Human, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 1,500pmol/min/ug, and was obtained by measuring the decrease of NADP in absorbance at 340nm resulting from NADPH at pH 7.0 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Aldo-Keto Reductase 1B10/AKR1B10

Sujet: AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E. coli and purified by using conventional chromatography.

Poids moléculaire: 36 kDa (316aa) confirmed by MALDI-TOF

NCBI Accession: NP_064695