NFNB (AA 1-217) protein (His tag)

N° du produit ABIN667164

Cette protéine Recombinant est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour NFNB (AA 1-217) protein (His tag) (ABIN667164)

Antigène: NFNB

Type de proteíne: Recombinant

Origine: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 % by SDS - PAGE

Détail du produit

Attributs du protein: AA 1-217

Purification/Conjugué: His tag

Attributs du produit: NFNB, 1-217aa, E.coli, His tag, E.coli

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol

Stock: 4 °C

Détail du antigène

Antigène: NFNB

Sujet: NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive. NCBI no.: NP_415110

Poids moléculaire: 26.0 kDa (237aa) confirmed by MALDI-TOF