NfsB (AA 1-217) protein (His tag)

N° du produit ABIN667164

Cette protéine Recombinant est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour NfsB (AA 1-217) protein (His tag) (ABIN667164)

Antigène: NfsB

Type de proteíne: Recombinant

Origine: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-217

Purification/Conjugué: His tag

Séquence: MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV

Attributs du produit: NFNB, 1-217aa, E.coli, His tag, E.coli

Purification: > 95% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 50 mM Nacl, 1 mM DTT, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: NfsB

Sujet: NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5- (aziridin-1-yl) -2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E. coli NFNB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poids moléculaire: 26.0 kDa (237aa) confirmed by MALDI-TOF

NCBI Accession: NP_415110